Role of Central Nervous System Periostin in Cerebral Ischemia

• DOI: 10.1161/strokeaha.111.636662
• Publication Date: 2012-02-03T07:13:07Z
• Authors: Munehisa Shimamura; Yoshiaki Taniyama; Naruto Katsuragi; Nobutaka Koibuchi; Mariko Kyutoku; Naoyuki Sato; Mohammad Allahtav...; Kouji Wakayama; Hironori Nakagami; Ryuichi Morishita
• Keywords: Periostin; Neurite

Although periostin, an extracellular matrix glycoprotein, plays pivotal roles in survival, migration, and regeneration various cells, its expression function the brain are still unknown. Here, we investigated role of periostin ischemic brain.Expression full-length (periostin 1 [Pn1]) splicing variant lacking exon 17 2 [Pn2]) was examined by real-time reverse transcription polymerase chain reaction (RT-PCR), Western blotting, immunohistochemical staining male C57BL6/J mice. The actions were adult primary neuronal culture a transient middle cerebral artery occlusion (tMCAo) model.Expression Pn2, but not Pn1, mRNA markedly changed after tMCAo. Pn2 decreased core at 3 hours ischemia. At 24 hours, significantly increased both peri-ischemic regions. Periostin mainly observed neurons normal brain. However, temporarily region, astrocytes around endothelial cells Of importance, intracerebroventricular injection resulted significant reduction infarct volume tMCAo associated with phosphorylation Akt. Also, Pn2-treated mice survived longer until week inhibited death under hypoxia stimulated neurite outgrowth.Here, demonstrated that expressed brain, exogenous exhibited neuroprotective effects accelerated outgrowth. Additional studies on may provide new insights into treatment stroke.