Altered Expression of Long Noncoding RNAs in Blood After Ischemic Stroke and Proximity to Putative Stroke Risk Loci

• DOI: 10.1161/strokeaha.116.013869
• Publication Date: 2016-11-14T11:07:26Z
• Authors: Cheryl Dykstra-Ai...; Glen C. Jickling; Bradley P. Ander; Natasha Shroff; Xinhua Zhan; DaZhi Liu; Heather Hull; Miles Orantia; Boryana S. Stamova; Frank R. Sharp
• Keywords: Stroke; Sexual dimorphism

Background and Purpose— Although peripheral blood mRNA micro-RNA change after ischemic stroke, any role for long noncoding RNA (lncRNA), which comprise most of the genome have been implicated in various diseases, is unknown. Thus, we hypothesized that lncRNA expression also changes stroke. Methods— was assessed 266 whole-blood samples drawn once per individual from patients with stroke matched vascular risk factor controls. Differential by ANCOVA ( P <0.005; fold change>|1.2|), principal components analysis, hierarchical clustering on a derivation set (n=176) confirmed validation (n=90). Poststroke temporal were using confounding correction partial correlation time since event >|0.4|). Because sexual dimorphism exists analyses performed each sex separately. Results— A total 299 lncRNAs differentially expressed between control males, whereas 97 females. Significant detected 49 men 31 women. Some mapped close to genomic locations previously identified putative stroke-risk genes, including lipoprotein, lipoprotein(a)-like 2, ABO (transferase A, α1-3-N-acetylgalactosaminyltransferase; transferase B, α1-3-galactosyltransferase) group, prostaglandin 12 synthase, α-adducins. Conclusions— This study provides evidence altered sexually dimorphic compared controls suggests potential biomarker development. regulated could regulate some genes.