TREM-1 Expression in Tumor-associated Macrophages and Clinical Outcome in Lung Cancer

• DOI: 10.1164/rccm.200704-641oc
• Publication Date: 2007-12-21T02:10:44Z
• Authors: Chao-Chi Ho; Wei-Yu Liao; Cheng-Yi Wang; Yin-Hsiu Lu; Hsin-Yi Huang; Hsuan-Yu Chen; Wing-Kai Chan; Huei-Wen Chen; Pan-Chyr Yang
• Keywords: Proinflammatory cytokine

Rationale: Triggering receptor expressed on myeloid cells (TREM)-1 is a molecule crucial for the triggering and amplification of inflammatory response new biomarker sepsis. Tumor-associated macrophages inflammation in tumor microenvironment are also involved cancer progression.Objectives: To determine role TREM-1 tumor-associated macrophage progression.Methods: Using ELISA Western blot, we measured soluble levels 65 pleural effusions various etiologies. We evaluated TREM-1–positive by immunocytochemistry malignant effusion lung versus adjacent normal tissue surgical specimens from 68 patients with non–small cell (NSCLC). expression was correlated patient survival. primary isolated peripheral blood cocultured lines determined quantitative real-time reverse transcriptase–polymerase chain reaction.Measurements Main Results: Soluble increased NSCLC. Lung could directly up-regulate proinflammatory cytokine (tumor necrosis factor-α, IL-1β) coculture experiments. Increased NSCLC were associated reduced disease-free (P = 0.011) overall survival 0.004). Multivariate Cox regression analysis indicated that an independent predictor (hazard ratio, 2.72; 95% confidence interval, 1.33–5.57; P 0.006).Conclusions: Cancer can patients' macrophages. recurrence poor may play important progression.