Gene Expression Profiles duringIn VivoHuman Rhinovirus Infection

• DOI: 10.1164/rccm.200805-670oc
• Publication Date: 2008-07-25T02:06:08Z
• Authors: David Proud; Ronald B. Turner; Birgit Winther; Shahina Wiehler; Jay P. Tiesman; Tim D. Reichling; Kenton D. Juhlin; Andy W. Fulmer; Begonia Y. Ho; Amy A. Walanski; Cathy L. Poore; Haruko Mizoguchi; Lynn Jump; Marsha L. Moore; Claudine K. Zukowski; Jeffrey W. Clymer
• Keywords: Rhinovirus

Human rhinovirus infections cause colds and trigger exacerbations of lower airway diseases.To define changes in gene expression profiles during vivo infections.Nasal epithelial scrapings were obtained before experimental infection, was evaluated by microarray. Naturally acquired infections, cultured human cells, short interfering RNA knockdown used to further evaluate the role viperin infections.Symptom scores viral titers measured subjects inoculated with or sham control, assessed 8 48 hours after inoculation. Real-time reverse transcription-polymerase chain reaction for rhinoviruses naturally mRNA levels cells. Rhinovirus-induced not observed but 11,887 transcripts significantly altered 2 days postinoculation. Major groups up-regulated genes included chemokines, signaling molecules, interferon-responsive genes, antivirals. Viperin examined also increased as well cells infected intact, replication-deficient, rhinovirus. Knockdown replication cells.Rhinovirus infection alters many associated immune response, including chemokines The data provide insights into host response identify potential novel targets evaluation.