Role of Lung Apolipoprotein A-I in Idiopathic Pulmonary Fibrosis

• DOI: 10.1164/rccm.200905-0659oc
• Publication Date: 2010-05-13T02:16:39Z
• Authors: Tae Hoon Kim; Yoo Hoon Lee; Kyung Hun Kim; Shin Hwa Lee; Ji Yeon Cha; Eun Kyoung Shin; Seok Jung; An Soo Jang; Sung Woo Park; Soo Taek Uh; Young Hoon Kim; Jai Soung Park; Hwa Gyoun Sin; Wook Youm; Eun Suk Koh; Sun Young Cho; Young Ki Paik; Tai Youn Rhim; Choon Sik Park
• Keywords: Male; Proteomics; 0301 basic medicine; 0303 health sciences; Apolipoprotein A-I; Pulmonary Fibrosis; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Middle Aged; Idiopathic Pulmonary Fibrosis; 3. Good health; Bleomycin; Disease Models, Animal; Mice; 03 medical and health sciences; Animals; Humans; Electrophoresis, Gel, Two-Dimensional; Female; RNA, Messenger; Bronchoalveolar Lavage Fluid; Lung; Aged; Foam Cells

Idiopathic pulmonary fibrosis (IPF) is caused by alterations in expression of proteins involved multiple pathways, including matrix deposition, inflammation, injury, and repair.To understand the pathogenic changes lung protein IPF to evaluate apolipoprotein (Apo) A-I as a candidate therapeutic molecule.Two-dimensional electrophoresis was adopted for differential display proteomics. Reverse-transcriptase polymerase chain reaction, Western blotting, immunohistochemical staining, ELISA were performed identification quantitative measurement Apo bronchoalveolar lavage fluids from subjects with experimental bleomycin-induced mice.Sixteen spots showed differences relative intensity between (n = 14) healthy control 8). Nano liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed increase haptoglobulin decrease alpha(1)-antitrypsin, alpha(1)-antichymotrypsin, macrophage capping protein, angiotensinogen, hemoglobin B, A-I, clusterin, disulfide isomerase A3, immunoglobulin, complement C4A compared normal (P 0.006-0.044). concentrations lower 28) than 18; P < 0.01). In bleomycin-treated mice, BALF that sham-treated animals. Immunohistochemical analysis positive staining on intraalveolar macrophages epithelial cells lungs. Intranasal treatment reduced increases number inflammatory collagen deposition mice dose-dependent manner.Alterations several antiinflammatory lungs may be related pathogenesis IPF, local very effective against development injury fibrosis.