Role of Lung Apolipoprotein A-I in Idiopathic Pulmonary Fibrosis

Male Proteomics 0301 basic medicine 0303 health sciences Apolipoprotein A-I Pulmonary Fibrosis Blotting, Western Enzyme-Linked Immunosorbent Assay Middle Aged Idiopathic Pulmonary Fibrosis 3. Good health Bleomycin Disease Models, Animal Mice 03 medical and health sciences Animals Humans Electrophoresis, Gel, Two-Dimensional Female RNA, Messenger Bronchoalveolar Lavage Fluid Lung Aged Foam Cells
DOI: 10.1164/rccm.200905-0659oc Publication Date: 2010-05-13T02:16:39Z
ABSTRACT
Idiopathic pulmonary fibrosis (IPF) is caused by alterations in expression of proteins involved multiple pathways, including matrix deposition, inflammation, injury, and repair.To understand the pathogenic changes lung protein IPF to evaluate apolipoprotein (Apo) A-I as a candidate therapeutic molecule.Two-dimensional electrophoresis was adopted for differential display proteomics. Reverse-transcriptase polymerase chain reaction, Western blotting, immunohistochemical staining, ELISA were performed identification quantitative measurement Apo bronchoalveolar lavage fluids from subjects with experimental bleomycin-induced mice.Sixteen spots showed differences relative intensity between (n = 14) healthy control 8). Nano liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed increase haptoglobulin decrease alpha(1)-antitrypsin, alpha(1)-antichymotrypsin, macrophage capping protein, angiotensinogen, hemoglobin B, A-I, clusterin, disulfide isomerase A3, immunoglobulin, complement C4A compared normal (P 0.006-0.044). concentrations lower 28) than 18; P < 0.01). In bleomycin-treated mice, BALF that sham-treated animals. Immunohistochemical analysis positive staining on intraalveolar macrophages epithelial cells lungs. Intranasal treatment reduced increases number inflammatory collagen deposition mice dose-dependent manner.Alterations several antiinflammatory lungs may be related pathogenesis IPF, local very effective against development injury fibrosis.
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