The Clinical and Environmental Determinants of Airway Transcriptional Profiles in Allergic Asthma

• DOI: 10.1164/rccm.201108-1503oc
• Publication Date: 2012-01-13T07:25:44Z
• Authors: Ivana V. Yang; John Tomfohr; Jaspal Singh; Catherine M. Foss; Harvey E. Marshall; Loretta G. Que; Erin McElvania-Te...; Sarita Florence; John S. Sundy; David A. Schwartz
• Keywords: Atopy

Gene expression profiling of airway epithelial and inflammatory cells can be used to identify genes involved in environmental asthma.Airway epithelia were obtained via bronchial brush bronchoalveolar lavage (BAL) from 39 subjects comprising three phenotypic groups (nonatopic nonasthmatic, atopic asthmatic) 4 hours after instillation LPS, house dust mite antigen, saline distinct subsegmental bronchi. RNA transcript levels assessed using whole genome microarrays.Baseline (saline exposure) differences gene related airflow obstruction (C3, ALOX5AP, CCL18, others), serum IgE (innate immune focal adhesion pathway) allergic-asthmatic phenotype (complement genes, histone deacetylases, GATA1 transcription factor) cells. LPS stimulation resulted pronounced transcriptional response across all both BAL cells, with strong association nuclear factor-κB IFN-inducible as well signatures other factors (NRF2, C/EBP, E2F1) proteins. No profile was observed the asthma atopy phenotype. Finally, although no consistent changes antigen stimulation, we subtle (e.g., GATA2) phenotype.Our results indicate that among individuals allergic asthma, are influenced by exposures.