Autophagy Primes Neutrophils for Neutrophil Extracellular Trap Formation during Sepsis

• DOI: 10.1164/rccm.201603-0596oc
• Publication Date: 2017-03-30T21:20:15Z
• Authors: So Young Park; Sanjeeb Shrestha; Young-Jin Youn; Jun-Kyu Kim; Shin-Yeong Kim; Hyun Jung Kim; So-Hee Park; Won-Gyun Ahn; Shin Kim; Myung Goo Lee; Ki-Suck Jung; Yong Bum Park; Eun-Kyung Mo; Yousang Ko; Suh-Young Lee; Younsuck Koh; Myung Jae Park; Dong-Keun Song; Chang-Won Hong
• Keywords: Neutrophil Extracellular Traps; Pathogenesis

Neutrophils are key effectors in the host's immune response to sepsis. Excessive stimulation or dysregulated neutrophil functions believed be responsible for sepsis pathogenesis. However, mechanisms regulating functional plasticity of neutrophils during have not been fully determined.We investigated role autophagy patients with community-acquired pneumonia.Neutrophils were isolated from and stimulated phorbol 12-myristate 13-acetate (PMA). The levels reactive oxygen species generation, extracellular trap (NET) formation, granule release, autophagic status evaluated. effect augmentation was further evaluated a mouse model sepsis.Neutrophils who survived showed an increase induction, primed NET formation subsequent PMA stimulation. In contrast, did survive decreased induction healthy vice versa. sepsis, improved survival via NET-dependent mechanism.These results indicate that primes increased which is important proper effector Our study provides insights into