Bottomless barrel-sponge species in the Indo-Pacific?

• DOI: 10.11646/zootaxa.4136.2.11
• Publication Date: 2016-07-06T01:37:40Z
• Authors: EDWIN SETIAWAN; NICOLE J. DE VOOGD; JOHN N.A. HOOPER; GERT WÖRHEIDE; DIRK ERPENBECK
• Keywords: Bilateria; Amplicon

The use of nuclear markers, in addition to traditional mitochondrial helps clarify hidden patterns genetic structure natural populations (Palumbi & Baker, 1994). This is particularly evident among demosponges that possess slow evolutionary rates compared Bilateria, where intron markers can aid the understanding shallow level phylogenetic relationships (Shearer et al., 2002). Ideally, these (i) are well-conserved across different lineages, (ii) produce amplicons holding a number sites with sufficient variability answer relevant question, (iii) derive from single copy genes (see review Zhang Hewitt, 2003). A popular method amplify uses EPIC (Exon-Primed, Intron-Crossing) primers anneal more conserved flanking exon regions and subsequently bridge during amplification