Antibody Responses Against Proteins of Different Molecular Weight Following Inoculation of Formalin Inactivated <i>Acinetobacter baumannii</i> in Mice

• DOI: 10.11648/j.iji.20251301.11
• Publication Date: 2025-04-02T03:02:11Z
• Authors: Kohinur Hasan; SM Shamsuzzaman; Mohammad Rashid; Farhana Parveen
• Keywords: Acinetobacter baumannii

<i>Acinetobacter baumannii</i> is one of the most significant threats to global public health with a seemingly limitless capacity for antimicrobial resistance. Developing preventive vaccines combat these pathogens can provide long-term protective immunity. This study was conducted evaluate specific protein against which antibody produced in serum and splenic cell culture supernatant after vaccination mice. The vaccine used formaldehyde-inactivated whole-cell multidrug resistant (MDR) <i>A. baumannii.</i> MDR baumannii</i>, isolated from different samples, inoculated intradermally experimental Serum collected tail blood on days 13, 27 41 following 1st immunization. Two weeks 3<sup>rd </sup>immunization, mice were challenged live intraperitoneally observed 14 days. Sera mouse spleens collected. antigen sonicated Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis performed. Protective antibodies that bind antigens evaluated by ELISA. Survival rates be 100% at post-challenge among immunized pre- immunizations showed considerably greater optical density (OD) values IgG than control Although are developed several molecular weights, fraction 30-40 kDa higher OD value. Formalin-inactivated intradermal immunization proteins weights. Proteins weights could as candidates prevent infection.