Interleukin-4-dependent pulmonary eosinophil infiltration in a murine model of asthma.

• DOI: 10.1165/ajrcmb.10.5.8179915
• Publication Date: 2013-04-04T02:29:38Z
• Authors: N W Lukacs; R M Strieter; S W Chensue; S L Kunkel
• Keywords: Infiltration (HVAC); Cellular infiltration; Monocyte

The establishment of animal models asthma is critical to elucidate the mechanisms involved in pathogenesis disease. In present study, we have used a parasite antigen from Schistosoma mansoni eggs, which induces TH2 response, elicit pulmonary inflammatory reaction that resolves after 3 4 days. Histologic examination lungs soluble egg antigens (SEA) or saline vehicle-challenged mice demonstrated large influx cells antigen- but not mice, thus demonstrating an antigen-specific reaction. A characteristic eosinophils could be detected as early 8 h, with significant increases at 24 72 h challenge. An assessment bronchial alveolar lavage (BAL) fluid dominant neutrophil infiltration subsequent decrease background by 48 h. addition, peak monocyte occurred and eosinophil extravasation into airway was shown leukocyte infiltrates interstitium dispersed lung preparations again challenge, lymphocyte Examination interleukin-4 (IL-4) production BAL presence IL-4 levels peaking between no detectable mice. Mice treated anti-IL-4 antibodies tenfold challenge reduction total cellularity.(ABSTRACT TRUNCATED AT 250 WORDS)