Differential Response of Dendritic Cells to Human Metapneumovirus and Respiratory Syncytial Virus

• DOI: 10.1165/rcmb.2005-0287oc
• Publication Date: 2005-11-12T01:34:11Z
• Authors: Antonieta Guerrer...; Antonella Casola; Giovanni Suarez; Xiang Yu; LeAnne Spetch; Mark E. Peeples; Roberto P. Garofalo
• Keywords: Metapneumovirus; Pneumovirus

Dendritic cells (DCs) play a pivotal role in shaping antiviral immune responses the respiratory tract. Human metapneumovirus (hMPV) is recently identified pathogen and like its better known relative, syncytial virus (RSV), has been increasingly recognized as major cause of morbidity infants elderly persons. In present study, we examined susceptibility well cellular human DCs to hMPV compared with RSV. Monocyte-derived (moDCs) were susceptible infection by both viruses, but only RSV was able induce productive release viral progeny. Despite fact that resulted phenotypic maturation moDCs, shown upregulation cell surface markers antigen-presenting molecules (MHC I II, CD80, CD83, CD86, CD38), RSV-infected moDCs showed severely impaired capacity stimulate CD4+ T proliferation. Compared hMPV, more potent inducer inflammatory immunomodulatory cytokines, including TNF-alpha, IL-6, IL-1beta, IL-10, IL-12p70 plasmacytoid dendritic (pDCs). On other hand, not RSV, trigger production IFN-alpha while viruses strongly induced pDCs. Finally, strikingly suppressed or pDCs stimulated synthetic dsRNA CpG-ODN, respectively. The findings provide novel evidence differentially activate may use distinct mechanisms interfere host innate adaptive responses.