Transient Receptor Potential Ankyrin 1 Channels Modulate Inflammatory Response in Respiratory Cells from Patients with Cystic Fibrosis

• DOI: 10.1165/rcmb.2016-0089oc
• Publication Date: 2016-06-09T18:23:03Z
• Authors: Paola Prandini; Francesco De Logu; Camilla Fusi; Lisa Provezza; Romina Nassini; Giulia Montagner; Serena Materazzi; Silvia Munari; Eliana Gilioli; Valentino Bezzerri; Alessia Finotti; Ilaria Lampronti; Anna Tamanini; Maria Cristina De...; Giuseppe Lippi; Carla M. Ribeiro; Alessandro Rimessi; Paolo Pinton; Roberto Gambari; Pierangelo Geppetti; Giulio Cabrini
• Keywords: Proinflammatory cytokine

Pseudomonas aeruginosa colonization, prominent inflammation with massive expression of the neutrophil chemokine IL-8, and luminal infiltrates neutrophils are hallmarks chronic lung disease in patients cystic fibrosis (CF). The nociceptive transient receptor potential ankyrin (TRPA) 1 calcium channels have been recently found to be involved nonneurogenic inflammation. Here, we investigate role TRPA1 CF respiratory inflammatory models vitro. Expression was evaluated tissue sections cells by immunohistochemistry immunofluorescence. Epithelial cell lines (A549, IB3-1, CuFi-1, CFBE41o-) primary from were used to: (1) check function modulation, Fura-2 imaging; (2) down-modulate expression, pharmacological inhibitors (HC-030031 A-967079) small interfering RNA silencing; (3) assess effect down-modulation on release cytokines upon exposure proinflammatory challenges, quantitative RT-PCR 27-protein Bioplex assay. expressed pseudostratified columnar epithelium facing bronchial lumina exposed bacteria, where IL-8 is coexpressed. Inhibition results a relevant reduction several cytokines, including IL-1β TNF-α, epithelial P. supernatant mucopurulent material derived chronically infected airways CF. In conclusion, regulating extent airway driven cells.