HIV-Nef Protein Persists in the Lungs of Aviremic Patients with HIV and Induces Endothelial Cell Death

• DOI: 10.1165/rcmb.2018-0089oc
• Publication Date: 2018-10-15T18:59:00Z
• Authors: Sarvesh Chelvanambi; Natalia V. Bogatc...; Mariola Bednorz; Stuti Agarwal; Bernhard Maier; Nathan J. Alves; Wei Li; Farooq Syed; Manal M. Saber; Noelle Dahl; Hongyan Lu; Richard B. Day; Patricia Smith; Paul Jolicoeur; Qigui Yu; Navneet K. Dhillon; Norbert Weissmann; Homer L. Twigg III; Matthias Clauss
• Keywords: 0301 basic medicine; Apoptosis; HIV Infections; Cell Line; Jurkat Cells; Mice; 03 medical and health sciences; Cell Line, Tumor; endothelial; Animals; Humans; Endothelium; Lung; Cells, Cultured; Cell Death; Macrophages; Endothelial Cells; RNA-Binding Proteins; Neoplasm Proteins; 3. Good health; EMAPII; emphysema; HEK293 Cells; Pulmonary Emphysema; Leukocytes, Mononuclear; Cytokines

It remains a mystery why HIV-associated end-organ pathologies persist in the era of combined antiretroviral therapy (ART). One possible mechanism is the continued production of HIV-encoded proteins in latently HIV-infected T cells and macrophages. The proapoptotic protein HIV-Nef persists in the blood of ART-treated patients within extracellular vesicles (EVs) and peripheral blood mononuclear cells. Here we demonstrate that HIV-Nef is present in cells and EVs isolated from BAL of patients on ART. We hypothesize that HIV-Nef persistence in the lung induces endothelial apoptosis leading to endothelial dysfunction and further pulmonary vascular pathologies. The presence of HIV-Nef in patients with HIV correlates with the surface expression of the proapoptotic endothelial-monocyte-activating polypeptide II (EMAPII), which was implicated in progression of pulmonary emphysema via mechanisms involving endothelial cell death. HIV-Nef protein induces EMAPII surface expression in human embryonic kidney 293T cells, T cells, and human and mouse lung endothelial cells. HIV-Nef packages itself into EVs and increases the amount of EVs secreted from Nef-expressing T cells and Nef-transfected human embryonic kidney 293T cells. EVs from BAL of HIV+ patients and Nef-transfected cells induce apoptosis in lung microvascular endothelial cells by upregulating EMAPII surface expression in a PAK2-dependent fashion. Transgenic expression of HIV-Nef in vascular endothelial-cadherin+ endothelial cells leads to lung rarefaction, characterized by reduced alveoli and overall increase in lung inspiratory capacity. These changes occur concomitantly with lung endothelial cell apoptosis. Together, these data suggest that HIV-Nef induces endothelial cell apoptosis via an EMAPII-dependent mechanism that is sufficient to cause pulmonary vascular pathologies even in the absence of inflammation.