Preparation, Characterization and Anti-Glioma Effects of Docetaxel-Incorporated Albumin-Lipid Nanoparticles

• DOI: 10.1166/jbn.2015.2076
• Publication Date: 2015-07-30T00:05:05Z
• Authors: Huile Gao; Shijie Cao; Zhi Yang; Shuang Zhang; Qizhi Zhang; Xinguo Jiang
• Keywords: Zeta potential

Poor aqueous solubility is a serious problem for most chemotherapeutics. Docetaxel (DTX), an inhibitor of microtubule depolymerization, frequently used to treat many malignancies. However, the surfactant in its commercial preparation (Taxotere) has proven problematic clinical use because it been associated with several side effects. By utilizing high DTX-loading property albumin, new formulation, DTX-incorporated albumin-lipid nanoparticles (DNPs), was prepared and evaluated. DTX bound albumin vitro dispersed by egg yolk lecithin. The DNP particle size 110.1 nm, while average zeta potential -2.95 mV. median lethal dose DNPs 180.6 mg/kg, which 75.3% higher than that Taxotere. could effectively inhibit proliferation cell lines induce apoptosis. In vivo imaging suggested localize accumulate at glioma site, likely due enhanced permeation retention effects DNPs. These pharmacological experiments further confirmed can tumor growth, prolong survival time mice gliomas levels conclusion, this novel formulation (DNPs) displayed lower toxicity superior anti-glioma effect relative standard preparations.