Elaboration of Sterically Stabilized Liposomes for <I>S</I>-Nitrosoglutathione Targeting to Macrophages

• DOI: 10.1166/jbn.2016.2130
• Publication Date: 2015-10-13T21:39:40Z
• Authors: R. Diab; A. S. Virriat; C. Ronzani; S. Fontanay; S. Grandemange; A. Elaissari; B. Foliguet; P. Maincent; P. Leroy; R. E. Duval; B. H. Rihn; O. Joubert
• Keywords: S-Nitrosoglutathione; Nanocarriers

S-nitrosoglutathione (GSNO) is a potential therapeutic for infectious disease treatment because of its pivotal role in macrophage-mediated inflammatory responses and host defense addition to direct antibacterial activities. In this study, sterically stabilized cationic liposomes (SSCL) anionic (SSAL) were developed as nanocarriers macrophage targeting. Elaborated characterized terms size, zeta potential, morphology, encapsulation efficiency, vitro drug release behavior cytotoxicity. Their versatility targeting monocytes/macrophages was determined by confocal laser scanning microscopy transmission electron microscopy. Flow cytometry revealed that cellular uptake both SSCL SSAL governed several endocytic clathrin- caveolae-dependent mechanisms. Quantitative assessments intracellular nitric oxide demonstrated highly efficient GSNO-loaded twenty-fold higher than GSNO-free molecules. displayed strong bacteriostatic effects on Staphylococcus aureus Pseudomonas aeruginosa, which can be involved pulmonary diseases. These results reveal the liposomal GSNO an anti-infective due capacity effects.