Micron/Submicron Hybrid Topography of Titanium Surfaces Influences Adhesion and Differentiation Behaviors of the Mesenchymal Stem Cells

• DOI: 10.1166/jbn.2017.2335
• Publication Date: 2017-05-26T12:16:41Z
• Authors: Peng Chen; Toshihiro Aso; Ryuichiro Sasaki; Yusuke Tsutsumi; Maki Ashida; Hisashi Doi; Takao Hanawa
• Keywords: Vinculin; Biomaterial; Surface Modification; Micropatterning

To clarify the effects of micron/submicron hybrid topography on cell morphology and functionalization, we investigated adhesion differentiation human mesenchymal stem cells (hMSCs) to titanium (Ti) surfaces with three different topographies: micron, submicron, grooves created using a femtosecond laser. hMSCs cultured Ti specimens showed high alignment micron after 6 h incubation, whereas attached submicron were elongated. An examination vinculin-positive plaques indicated that affected cellular by modifying initial polarization, extension. A superposition effect was evidenced highly aligned elongated grown surface, which promoted osteogenic chondrogenic differentiation. These findings provide basis for design novel biomaterial can control specific functions.