Construction of a New Dual-Responsive Nano-Drug Delivery System for Matrix Metalloproteinases and Adenosine Triphosphate in Ovarian Cancer Using Nanomicelles

• DOI: 10.1166/jbn.2022.3303
• Publication Date: 2022-06-18T04:42:13Z
• Authors: Guocheng Li; Wenwen Xu; Yong Shi; Mengzhu Chen; Danhong Peng
• Keywords: Doxorubicin Hydrochloride; Viability assay

In this manuscript we constructed a dual-responsive nano-drug delivery system for matrix metalloproteinases and ATP in ovarian cancer microenvironment. The nanomicelle PCL-DNA/DOX-Peptide-PEG was prepared by intercalating doxorubicin hydrochloride between C G base pairs of DNA double helix structure. Another ATP-responsive PCL-DNA/DOX-PEG prepared. Then analyzed the characterization nanomicelles (particle size, potential, surface morphology, etc.) drug loading binding release behavior. addition, effect on viability mouse epithelial tumor cell ID-8 detected CCK-8 method. assay that different concentrations carrier had no difference proliferation cells, survival rate cells DOX preparations statistically significant same results were observed cytotoxicity comparison. Confocal microscopy showed drug-loaded micelle group concentrated nucleus, while free cytoplasm. took up micelles faster. semi-quantitative analysis uptake with treatments extremely statistical differences. conclusion, self-assembled is novel anti-tumor agent, expected to have good tissue penetration ability low toxicity.