Effects of Ambient Relative Humidity and Solvent Properties on the Electrospinning of Pure Hyaluronic Acid Nanofibers

• DOI: 10.1166/jnn.2013.7197
• Publication Date: 2013-05-28T08:03:20Z
• Authors: Shenglian Yao; Xiumei Wang; Xi Liu; Ronghan Wang; Changsheng Deng; Fuzhai Cui
• Keywords: Electrospinning

Nanofibers exist ubiquitously in natural extracellular matrix (ECM) of all kinds human tissues forming hydrated interwoven network. Electrospinning nanotechnology has been proven to be a powerful technique fabricate controllable nanofibers mimicking the ECM structures. Hyaluronic acid (HA), as critical component ECM, widely used tissue engineering and regenerative medicine. In this study, pure HA with average diameter 33 +/- 5 nm, 59 12 79 nm 113 19 were successfully prepared using different electrospinning parameters. The effect ambient relative humidity on electrospinnability was investigated for first time detail, which one most important factors control morphology beside solution properties. A value defined observed, only below similar diameters morphologies could obtained. When higher than value, started dissolving at cross points even fused together spreading layer. Moreover, small amount N, N-Dimethylformamide (DMF) found required promote by mixing water solvents. With increase DMF content, surface tension decreased significantly, thought benefit stable Taylor cone fluid jet formation electrospinning. At same time, it should noted that conductivity also content solution, believed responsible increasing corresponding content. Controllable 100 have great promising developing novel nanobiomaterials applied