Quantitative Biokinetics and Systemic Translocation of Various Gold Nanostructures Are Highly Dependent on Their Size and Shape

• DOI: 10.1166/jnn.2014.8274
• Publication Date: 2014-02-15T04:05:24Z
• Authors: Jiajing Zhang; Xin Nie; Yinglu Ji; Ying Liu; Xiaochun Wu; Chunying Chen; Xiaohong Fang
• Keywords: Biodistribution; Bovine serum albumin; Nanorod; Surface charge; Nanoclusters

Gold nanostructures with promising applications in biomedical field have attracted great attention. However, some fundamental questions other than the development of novel should be elucidated before they can actually serve as biomedicines clinic. Bio-safety is one most important issues. Since numerous modifications (e.g., surface coating and composites) been designed on gold nanoparticles (GNPs) to extend their application, there would hundreds GNPs synthesized lab although prototypes (i.e., cluster, shell, rod, sphere, cage, star) are rather limited. Thus, present work we aim conduct our experiments only basic types GNPs--including nanocluster (GNC), nanorod (GNR) nanosphere (GNS), investigate biodistribution toxicities vivo, hope revealing rules which could further extended complicated situations. Bovine serum albumin (BSA) was coated at increase plasma stability same BSA help compare fate behaviors various vivo. After intravenous administration different an equal content element 0.5 mg/kg mice, samples were harvested a series time points. Biodistribution compared among process accumulation-retention-clearance each kind GNP also observed through quantification analysis by inductively coupled plasma-mass spectrometry (ICP-MS). The results showed, similar negative charge, size shape dominating vivo GNPs. Even between GNC hydrolyzed 7.1 3.2 nm, huge difference kidney accumulation observed. Totally, GNR GNS relative large preferred accumulate liver spleen whereas small tended kidney. resided hardly cleared out body till 28 d almost entirely eliminated prolonged time, not rapid reported previous work. In evaluated pathology observation blood biochemical revealed slight damage, basically associated pattern