A Microreactor System for Cultivation of <I>Haematococcus pluvialis</I> and Astaxanthin Production

• DOI: 10.1166/jnn.2015.9321
• Publication Date: 2014-09-24T21:20:30Z
• Authors: Ho Seok Kwak; Jaoon Young Hwan Kim; Sang Jun Sim
• Keywords: Microreactor; Pluvialis; Nile red; Light intensity

Development of efficient culture and monitoring system for cell growth production useful materials is required practical utilization microalgae. In the present study, we developed a PDMS-based microreactor efficient, rapid microalgae growth, carotenoid content under diverse conditions. Due to advantages PDMS, optimized conditions (light intensity, pH, nitrate depletion, carbon dioxide concentration) improving rate astaxanthin productivity in considerably less time compared conventional methods using flask or well plate. addition, found that there was strong linear correlation between fluorescence intensity stained by Nile red content, which can be utilized as high-throughput screening tool microfluidic systems. this vegetative Haematococcus pluvialis improved 60% chamber than produced up 362 mg/L optimal (300 µmol photon/m2/s light, 7% CO2 (v/v), pH 7.0) designed devices. This result shows provide effective means address development microalgal strains including H. bioprocess.