Effects of Ion Transport and Channel-Blocking Drugs on Aqueous Humor Formation in Isolated Bovine Eye

• DOI: 10.1167/iovs.02-0397
• Publication Date: 2003-02-24T21:25:04Z
• Authors: Mohammad Shahidullah; William S. Wilson; Maurice Yap; Chi-ho To
• Keywords: Bumetanide; DIDS; Carbonic anhydrase inhibitor; Chloride channel; Ciliary processes; Channel blocker; Amiloride

To investigate the role of active chloride secretion by ciliary epithelium in formation aqueous humor (AH), using vitro perfused eye.Bovine eyes collected from an abattoir were cannulated through ophthalmic artery and with oxygenated Krebs' solution at 37 degrees C. Aqueous (AHF) was measured fluorescein-dilution technique. Drugs added to perfusate and/or anterior chamber.NaK-adenosine triphosphatase (ATPase) inhibitor, ouabain (1.0 mM), produced a significant reduction AHF 46% 42% when stromal or side, respectively. When both sides it 61%. Bumetanide (0.1 specific inhibitor Na-K-2Cl cotransport, furosemide nonspecific anion transport 35% 45% reductions applied side. DIDS (0.001-0.1 which is believed inhibit Cl-HCO(3) exchanger, Na-HCO(3) cotransporter, channel, dose-dependent The inhibition 55% highest concentration used. 5-Nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB; 0.1 channel blocker nonpigmented cells, 25% Acetazolamide carbonic anhydrase 31% reduction.At least 60% AH formed bovine eyes. Transport anions (CE), particularly ion, plays crucial AHF.