A Murine Model of Dry Eye Induced by an Intelligently Controlled Environmental System

• DOI: 10.1167/iovs.07-0744
• Publication Date: 2008-04-01T22:29:25Z
• Authors: Wei Chen; Xiaobo Zhang; Jingna Zhang; Jinpeng Chen; Shiming Wang; Qinmei Wang; Jia Qu
• Keywords: Goblet cell; Desquamation

purpose. To establish a novel murine model of dry eye using an intelligently controlled environmental system (ICES). methods. Thirty BALB/c mice aged 4 to 6 weeks were housed in the ICES which relative humidity, airflow, and temperature maintained at 15.3% ± 3% (mean SD), 2.1 0.2 m/s, 21° 23°C, respectively, for 42 days. similar age normal environment controls (relative 60%–80%; no airflow; temperature, 21°–23°C). The ocular surfaces animals both groups analyzed before 3, 7, 14, 28, days after experiment aqueous tear production, corneal barrier function, conjunctival morphology, goblet cell density. level apoptosis on surface also was assessed active caspase-3 results. A low-humidity constantly by ICES. Animals this had decreased increased fluorescein staining, marked thinning accelerated desquamation apical epithelium compared with control eyes. Squamous metaplasia density developed Active highly expressed conclusions. biological morphologic changes induced are those humans. This appears upregulate surface.