Senescence cell–associated extracellular vesicles serve as osteoarthritis disease and therapeutic markers

• DOI: 10.1172/jci.insight.125019
• Publication Date: 2019-04-03T17:41:59Z
• Authors: Ok Hee Jeon; David R. Wilson; Cristina C. Clement; Sona Rathod; Christopher Cherry; Bonita Powell; Zhenghong Lee; Ahmad M. Khalil; Jordan J. Green; Judith Campisi; Laura Santambrogio; Kenneth W. Witwer; Jennifer H. Eliss...
• Keywords: Senescence; Extracellular vesicles

Senescent cells (SnCs) are increasingly recognized as central effector in age-related pathologies. Extracellular vesicles (EVs) potential cellular communication tools through which SnCs exert functions the local tissue environment. To test this hypothesis a medical indication that could be validated clinically, we evaluated EV production from enriched chondrocytes isolated human arthritic cartilage. increased dose-responsive manner concentration of increased. The EVs were capable transferring senescence to nonsenescent and inhibited cartilage formation by non-SnCs. microRNA (miR) profiles synovial fluid did not fully overlap with senescent chondrocyte profiles. effect SnC clearance was tested murine model posttraumatic osteoarthritis. miR protein changed after senolytic treatment but varied depending on age. In young animals, altered expression miR-34a, -30c, -125a, -24, -92a, -150, -186, correlated production. primary changes contents aged mice treatment, only reduced pain degeneration, immune related. sum, found may serve diagnostic for disease indicator therapeutic efficacy treatment.