ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies

• DOI: 10.1172/jci.insight.139163
• Publication Date: 2020-09-22T21:36:28Z
• Authors: Lihui Wang; Zhichao Ai; Tariq Khoyratty; Kristina Zec; Hayley L. Eames; Erinke van Grinsven; Alison Hudak; Susan Morris; David Ahern; Claudia Monaco; Evgeniy B. Eruslanov; Raashid Luqmani; Irina A. Udalova
• Keywords: Arteritis

Giant cell arteritis (GCA) is a common form of primary systemic vasculitis in adults, with no reliable indicators prognosis or treatment responses. We used single technologies to comprehensively map immune populations the blood patients GCA and identified CD66b+CD15+CD10lo/–CD64– band neutrophils CD66bhiCD15+CD10lo/–CD64+/bright myelocytes/metamyelocytes be unequivocally associated both clinical phenotype response treatment. Immature were resistant apoptosis, remained vasculature for prolonged period time, interacted platelets, extravasated into tissue surrounding temporal arteries GCA. discovered that immature generated high levels extracellular reactive oxygen species, leading enhanced protein oxidation permeability endothelial barrier an vitro coculture system. The same also detected other vasculitides. These findings link functions disease pathogenesis, establishing cellular signature suggesting different therapeutic approaches vascular inflammation.