Release of soluble receptors for tumor necrosis factor (TNF) in relation to circulating TNF during experimental endotoxinemia.

• DOI: 10.1172/jci115891
• Publication Date: 2008-02-28T22:42:12Z
• Authors: G A Spinas; U Keller; M Brockhaus
• Keywords: Ibuprofen

Serial plasma samples from human volunteers obtained after intravenous administration of Escherichia coli endotoxin were analyzed for the presence circulating soluble tumor necrosis factor receptors (sTNFR). A four- to fivefold increase type (p75) and B (p55) sTNFR was observed 3 h challenge. Pretreatment with ibuprofen before injection resulted in a slight (3.87 +/- 0.2 vs. 3.27 0.3 ng/ml) temporal shift sTNFR-A release concurrent marked augmentation TNF levels (603 118 338 56 pg/ml) as compared group without pretreatment. There significant correlation between peak individual probands (r = 0.52, P 0.04). On contrary, kinetics concentrations sTNFR-B identical both groups (7.38 0.69 7.44 0.33 no observed. The amount liberated upon challenge not sufficient block TNF-mediated cytotoxic effects. Our data indicate that vivo short exposure is regulated differently.