Expression of Apoptosis-associated Speck-like Protein Containing a Caspase Recruitment Domain, a Pyrin N-terminal Homology Domain-containing Protein, in Normal Human Tissues

• DOI: 10.1177/002215540104901009
• Publication Date: 2011-04-01T07:17:54Z
• Authors: Junya Masumoto; Shun'ichiro Tanig...; Jun Nakayama; Masaaki Shiohara; Eiko Hidaka; Tsutomu Katsuyama; Sumio Murase; Junji Sagara
• Keywords: Pyrin domain

Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is pyrin N-terminal homology (PYD)- and (CARD)-containing proapoptotic molecule. This molecule has also been identified as target of methylation-induced silencing (TMS)-1. We cloned the ASC cDNA by immunoscreening using an anti-ASC monoclonal antibody. In this study, we determined binding site antibody on analyzed expression in normal human tissues. was observed anterior horn cells spinal cord, trophoblasts placental villi, tubule epithelium kidney, seminiferous tubules Leydig testis, hepatocytes interlobular bile ducts liver, squamous epithelial tonsil skin, hair follicle, sebaceous eccrine glands peripheral blood leukocytes. colon, detected mature facing luminal side rather than immature located deeper crypts. These observations indicate that high levels are abundantly expressed leukocytes, which involved host defense against external pathogens well-differentiated cells, proliferation regulated.