Relevance of Two Genes in the Multidrug Resistance of Hepatocellular Carcinoma: In Vivo and Clinical Studies

• DOI: 10.1177/030089161009600115
• Publication Date: 2018-02-27T09:08:25Z
• Authors: Zhengang Sun; Zizhuo Zhao; Gaopeng Li; Shengli Dong; Zhiyong Huang; Lu Ye; Huifang Liang; Junyuan Qu; Xi Ai; Wanguang Zhang; Xiaoping Chen
• Keywords: Abcg2

Aims and background A former study evaluated the roles of four multidrug resistance-related proteins, namely resistance protein 1 (MDR1), breast cancer (BCRP), (MRP1), lung (LRP), in MDR mechanism resistant hepatoma HepG2/ADM cell line proposed that up-regulated MDR1 BCRP are responsible for hepatocellular carcinoma. This work aims to confirm assumption vivo clinical specimens. Methods First, chemotherapeutic subcutaneous tumor carcinoma samples post-transarterial chemoembolization (TACE) was determined by MTT, contrary HepG2 without TACE, respectively. Then, mRNA differential expression genes between tissues drug-sensitive were quantitatively investigated real-time RT-PCR enhanced chemiluminescence western blot analysis, Results 1) respectively amplified 38.3 20.1 fold tumors mice compared those mice, whereas they augmented 14.6 9.3 times TACE samples, TACE. 2) The presence also significantly higher than control group 3) expressions correlated their levels. Conclusions showed may be most important factors drug Moreover, positive correlation indicates easy prediction HCC possible inhibitive target at multi-levels.