Gene Analysis of Epstein-Barr Virus-Associated Lymphomas in Hu-PBL/SCID Chimeras

• DOI: 10.1177/030089161009600315
• Publication Date: 2018-02-27T09:14:23Z
• Authors: Runliang Gan; Xiaoli Xie; Jie He; Xiaomin Liu; Li Hong; Yunlian Tang; Fang Liu; Hailong Xie
• Keywords: Epstein-Barr Virus Infections; Herpesvirus 4, Human; Lymphoma; Chimera; Mice, SCID; Immunohistochemistry; Polymerase Chain Reaction; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; Proto-Oncogene Proteins c-myc; Mice; 03 medical and health sciences; 0302 clinical medicine; Proto-Oncogene Proteins c-bcl-2; Carcinogens; Animals; Humans; Lymphocytes; Tumor Suppressor Protein p53; In Situ Hybridization; Polymorphism, Single-Stranded Conformational; bcl-2-Associated X Protein

Aims and background The mechanisms of Epstein-Barr virus (EBV)-associated tumor development are incompletely understood. aim this study was to investigate the gene expression EBV-associated lymphomas in hu-PBL/SCID mice. Methods Human peripheral blood lymphocytes (hu-PBL) from EBV-seropositive donors were transplanted into severe combined immunodeficiency (SCID) In situ hybridization used detect EBV-encoded small RNA-1 (EBER1) tissues. Mutation TP53 exons 5–8 EBV-induced analyzed by PCR-SSCP. Immunohistochemical staining examine EBV products cellular oncoproteins. Results Twenty-one 29 mice developed tumors. EBER1 positive nuclei almost all cells. Immunohistochemistry showed LMP1, EBNA2 ZEBRA a number Immunohistochemically detectable p53 protein common (85.7%), but mutations identified only four cases (19.1%) lymphomas. Positivity rates C-myc, Bcl-2 Bax 100%, 95.2%, 90.5%, respectively, 21 Conclusions Our preliminary findings suggest that chimeras show infection, oncogenic viral genes, overexpression oncogenes. rare is commonly expressed