A Split-Mouth Study on Microbiological Profile in Clinical Healthy Teeth and Implants Related to Key Inflammatory Mediators

• DOI: 10.1177/039463201002300126
• Publication Date: 2017-01-10T19:24:48Z
• Authors: G. Schierano; G. Pejrone; J. Roana; D. Scalas; V. Allizond; G. Martinasso; M. Pagano; R.A. Canuto; A.M. Cuffini
• Keywords: Curette; Tannerella forsythia; Aggregatibacter actinomycetemcomitans

This split-mouth study investigated the correlation of qualitative and quantitative bacterial composition in dental plaque around clinically healthy periodontal peri-implant subgingival sites with levels selected pro- anti-inflammatory cytokines inflammatory infiltrate soft tissue surrounding a implant natural tooth same patient. Nine patients, all good health non-smokers, were studied. All patients highly motivated terms oral hygiene had teeth at least one implant. After three sessions professional care, clinical parameters recorded. A sample was harvested sterile curette from buccal side implants teeth. The samples cultured to quantify total microbiota number obligate facultative strains. Simultaneously, lingual/palatal aspect keratinized tissues biopsied for cytokine expression histomorphometric analysis. biopsies halved: real-time reverse transcriptase-polymerase chain reaction (PCR) performed detect active TNF-α, IL-1β, IL-8, TGF-β2 distribution, composition, quantification inflammation assessed parallel. harbored no periodontopathogens microbiological taken did not differ that No significant differences seen between both cytokines. Even histological examination showed epithelial changes, although slight perivascular lymphocytic infiltration some biopsies.