Endotoxin binding on capillary endothelium and myocyte plasma membranes in perfused rat heart

• DOI: 10.1177/096805199700400106
• Publication Date: 2008-01-25T19:57:02Z
• Authors: A. Bikhazl; Z. Nahle; S. Kreydiyyeh; R. Haddad; K. Bitar; G. Haddad; A. Abdelnoor
• Keywords: Dissociation constant

This work uses a novel heart-perfusion technique to measure [ 3 H]-lipopolysaccharide ([ H]-LPS) binding on capillary endothelium and myocyte cell membranes in Sprague-Dawley rats. Free or serum-containing Ringer-Lock buffer was infused at rate of 1 ml/min the presence 20 mM K + H]-LPS through an aortic cannula, effluent collected catheter introduced into right atrium cavity. The endothelial lining removed by CHAPS treatment expose cardiac surface. A physical model describing 1:1 stoichiometry with its receptors is proposed mathematical equations derived allow for calculation constants (k n ), reversal -n dissociation d residency time (τ). results showed that serum perfusate, slowed myocytes, but increased 3- 50-fold, respectively. Hence, myofiber may contain LPS can bind more strongly ligand association sCD14-like LBP-like molecules rat serum. Thus it postulated affinity receptor subtypes not strictly specifically dependent CD14 profile.