A Case Report: Evidence for Type 2 Bovine Viral Diarrhea Virus (BVDV)-Associated Disease in Beef Herds Vaccinated with a Modified-Live Type 1 BVDV Vaccine

• DOI: 10.1177/104063870001200312
• Publication Date: 2011-05-12T20:20:37Z
• Authors: H. van Campen; P. Vorpahl; S. Huzurbazar; J. Edwards; J. Cavender
• Keywords: Pestivirus

Fetal infections with bovine viral diarrhea virus (BVDV) may result in infertility, abortions, stillbirths, developmental anomalies, and weak calves.1 Infection of the fetus from 58 to 120 days gestation create persistently infected (PI) calves that shed continuously perpetuate herd infection.10,11 In beef herds, calf losses PI have a significant impact on productivity.14 Vaccination modified live (MLV) BVDV vaccines is commonly practiced Wyoming herds prevent reproductive due infection. However, majority isolates obtained at State Veterinary Laboratory (WSVL) are vaccinated herds. For example, 1996 was isolated 30 for which vaccination histories were available. Twenty-four had history 6 not BVDV. BVDV-associated diseases inactivated been reported.8,9 Here, we describe isolation type 2 where cows 1a MLV-BVDV vaccinea use serology detect infection retrospectively. fall 1995, 28 cow–calf located Weston County, Wyoming, unusually high infertility rates followed by spring 1996. 9 dams vaccine.a Gross findings aborted fetuses neonatal included hemorrhage subcutaneous tissues lower extremities, muscle, myocardium serosal surfaces multiple organs, serosanguinous fluid thorax abdominal cavities, lens opacities. Fourteen animal tissue samples 13 ranches submitted. These animals 5 fetuses, calves, 1 months age, yearling steer. Two autolyzed, remaining collected 15 minutes 18 hours postmortem. Virus isolations (VI) performed using 10% homogenates Bovarnick’s solution (0.218 M sucrose, 3 mM KH2PO4, 7.2 K2HPO4, 5.4 L-glutamic acid, 500 mg/liter streptomycin, 0.001% phenol red, pH 7.2) clarified centrifugation used inoculate primary embryonic testicle (BeT) cells previously tested found negative adventitial Inoculated BeT passed twice stained monoclonal antibody 20.10.6b fluorescein isothiocyanate-conjugated goat anti-mouse antibodyc as described.18 Blood jugular