Detection, sequencing, and tissue distribution of piscine orthoreovirus 2–like virus in diseased coho salmon in Alaska

• DOI: 10.1177/10406387241250119
• Publication Date: 2024-05-02T05:13:15Z
• Authors: Chrissy D. Eckstrand; Brandi K. Torrevi...; Rebecca M. Wolking; Daniel S. Bradway; Joetta Lynn Reno; Kathleen M. McMen...; Kevin R. Snekvik
• Keywords: 0301 basic medicine; 03 medical and health sciences

We performed a diagnostic disease investigation on cohort of coho salmon ( Oncorhynchus kisutch) fingerlings in Alaska exhibiting anorexia, gaping mouths, anemia, and increased mortality. Histologic examination revealed mild-to-severe myocardial degeneration lymphohistiocytic neutrophilic myocarditis, moderate splenic histiocytosis, mild renal histiocytosis. Piscine orthoreoviruses 1 3 were not detected by molecular methods, no other viruses could be cultured common fish cell lines. De novo assembly produced viral genome 10 linear segments with >80% homology to piscine orthoreovirus 2 (PRV2) encoding all 11 PRV2 proteins. An situ hybridization probe using RNAscope was developed against 697 nucleotides identified sequencing, which heart, spleen, gill, kidney, liver, blood, the lamina propria intestines. Our findings are supportive novel most closely related associated morbidity mortality northeastern Pacific.