Angiotensin II regulates collagen metabolism through modulating tissue inhibitor of metalloproteinase-1 in diabetic skin tissues

• DOI: 10.1177/1479164113485461
• Publication Date: 2013-06-25T00:26:45Z
• Authors: Meng Ren; Shaoyun Hao; Chuan Yang; Ping Zhu; Lihong Chen; Diaozhu Lin; Na Li; Li Yan
• Keywords: Type I collagen

We investigated the effect of angiotensin II (Ang II) on matrix metalloproteinase-1 (MMP-1)/tissue inhibitor (TIMP-1) balance in regulating collagen metabolism diabetic skin. Skin tissues from model were collected, and primary cultured fibroblasts treated with Ang receptor inhibitors before treatment. The type I (Coll I) III III) measured by histochemistry. expressions transforming growth factor-β (TGF-β), MMP-1, TIMP-1 propeptides types procollagens skin quantified using polymerase chain reaction (PCR), Western blot or enzyme-linked immunosorbent assay (ELISA). Collagen dysfunction was documented changed I/III ratio streptozotocin (STZ)-injected mice compared controls. This accompanied increased expression TGF-β, tissues. In fibroblasts, prompted synthesis increases procollagens, these inhibited losartan, an 1 (AT1) blocker, but not affected PD123319, 2 (AT2) antagonist. These findings present evidence that Ang-II-mediated changes productions MMP-1 occur via AT1 receptors a TGF-β-dependent mechanism.