Andrographolide Inhibits the NF-κB Signaling Pathway and Alleviates High-Fat Diet-Induced Hepatic Inflammation in a Mouse Model of Nonalcoholic Fatty Liver Disease

• DOI: 10.1177/1934578x241302020
• Publication Date: 2025-02-18T06:49:42Z
• Authors: Weiwen Hu; Yilin Chen; Wenhong Tan; Yirong Wang; Die Huang; Hao Yuan
• Keywords: Andrographolide; Nonalcoholic steatohepatitis

Background High-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) is a key link between several medical conditions and becoming major health concern affecting thousands of people globally. Andrographolide, an active ingredient extracted from Andrographis paniculate, has shown anti-inflammatory anti-fibrosis effects in recent studies. Objective This study aims to explore whether andrographolide can mitigate HFD-induced NAFLD the underlying mechanism. Methods We treated C57B/6 mice consuming high-fat (HFD) with varying concentrations andrographolide. Enzyme-linked immunosorbent assay (ELISA) was employed evaluate function indicators circulating blood. Haematoxylin Eosin Oil Red O staining were used assess inflammation infiltration lipid deposits tissues. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) immunoblotting analyze expression levels genes proteins. Results ELISA revealed that dose-dependently mitigated elevations glucose, alanine aminotransferase, aspartate aminotransferase Histological analysis indicated reduced deposition. RT-qPCR analyses showed inhibited overproduction tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, up-regulation p-p65 NLRP3 Conclusion Together, these results suggest treatment impairments, accumulation, pro-inflammatory cytokine overproduction, inflammatory responses by regulating NF-κB signaling pathway. However, it important note our are observational do not conclusively demonstrate necessity pathway's involvement NAFLD-alleviating highlights need for further validation at both gene protein through additional vitro vivo experiments.