Cytofluorometry of electromagnetically controlled cell dedifferentiation.

• DOI: 10.1177/27.1.86566
• Publication Date: 2011-04-01T05:19:10Z
• Authors: A Chiabrera; M Hinsenkamp; A A Pilla; J Ryaby; D Ponta; A Belmont; F Beltrame; M Grattarola; C Nicolini
• Keywords: Cell Nucleus; 0303 health sciences; Erythrocytes; Staining and Labeling; Electromagnétisme - analyse du signal; Erythrocytes -- cytology; Cytological Techniques; Rana pipiens; synthèse des protéines; Cell Differentiation; DNA; Sciences de l'ingénieur; Cell Nucleus -- analysis; Acridine Orange; 03 medical and health sciences; Electromagnetic Fields; DNA -- analysis; Animals; Biologie cellulaire; Female; Fluorometry; Anura; Acides nucléiques

Cellular morphology changes, which appear related to dedifferentiation (despecialization), have been produced in vitro the nucleated red blood cell of frog. This has achieved by controlled alterations electrochemical environment these living cells, both a selective modification ionic concentrations an isotonic amphibian Ringer solution, and electromagnetic induction pulsating current having specific waveform parameters. Laser flow microfluorometry shows that modified solution is able, per se, partially trigger process same time interval certain induced waveforms can significantly affect number cells so-called dedifferentiated state. It also found that, for given waveform, repetition rate appears significant effect on change. Preliminary automated image analysis smears suggests normal integrated optical density but different nuclear areas. In conclusion, it after initial trigger, early stage process, when move from state specialized function one less activity, unfolding their chromatin supercoil, not involving DNA synthesis. Then cytofluorometry allowed us identify, first time, fundamental modifications occur nucleus under exposure.