Characterization of a Cancer Stem Cell-Like Side Population Derived from Human Pancreatic Adenocarcinoma Cells

• DOI: 10.1177/548.6520
• Publication Date: 2018-04-17T07:41:27Z
• Authors: Shi-Neng Zhang; Feng-Ting Huang; Yi-Jun Huang; Wa Zhong; Zhong Yu
• Keywords: Side population; clone (Java method)

Aims and background To identify partially characterize the side population cells derived from three human pancreatic adenocarcinoma cell lines. Methods Side were sorted lines SW1990, Capan-2, BxPC-3 using flow cytometry then analyzed for proliferation, clone formation, differentiation, chemoresistance, invasive potential, tumorigenicity in a mouse model. Results Human carcinoma contain 2.7% ±0.35%, 3.6%± 1.2%, 2.8%± 0.8% cells, respectively. We further investigated cancer stem characteristics with moderately differentiated line SW1990. Flow analysis revealed that could differentiate into non-side exhibit differentiation potential. Using formation assay, shown to have higher proliferation than cells. Compared also more resistant gemcitabine, commonly used anti-cancer agent against carcinoma, invasive. Importantly, CD133 level was significantly The enhanced tumorigenecity confirmed male diabetic/severe combined immunodeficiency As few as 3 × 10 sufficient induce tumor model, compared 7 or unsorted Conclusions isolated harbor cell-like properties may be related potential therapeutic-resistance of adenocarcinoma. Free full text available at www.tumorionline.it