Insulin-like growth factor-1 attenuates apoptosis and protects neurochemical phenotypes of dorsal root ganglion neurons with paclitaxel-induced neurotoxicity in vitro

• DOI: 10.1179/1476830514y.0000000147
• Publication Date: 2014-08-19T15:13:02Z
• Authors: Cheng Chen; Xue Bai; Yanwen Bi; Guixiang Liu; Hao Li; Zhen Liu; Huaxiang Liu
• Keywords: Neurotoxicity; Dorsal root ganglion; Neurite; Viability assay; LY294002

Paclitaxel (PT)-induced neurotoxicity is a significant problem associated with successful treatment of cancers. Insulin-like growth factor-1 (IGF-1) neurotrophic factor and plays an important role in promoting axonal from dorsal root ganglion (DRG) neurons. Whether IGF-1 has protective effects on neurite growth, cell viability, neuronal apoptosis phenotypes DRG neurons PT-induced still unclear. In this study, primary cultured rat were used to assess the neurotoxicity. The results showed that PT exposure caused retraction dose-dependent manner. decrease viability increase ratio apoptotic cells which could be reversed by IGF-1. percentage calcitonin gene-related peptide immunoreactive (CGRP-IR) neurofilament (NF)-200-IR neurons, mRNA, protein levels CGRP NF-200 decreased significantly after PT. administration had CGRP-IR but not NF-200-IR Either extracellular signal-regulated kinase (ERK1/2) inhibitor PD98059 or phosphatidylinositol 3-kinase (PI3 K) LY294002 blocked effect imply may attenuate improve promote Moreover, these support neuroprotective exogenous distinct subpopulations responsible for skin sensation. might through ERK1/2 PI3 K/Akt signaling pathways. These findings provide experimental evidence alleviate induced