Decalcified Bone: Twenty Years of Successful Specimen Management

• DOI: 10.1179/his.1997.20.3.267
• Publication Date: 2014-01-09T17:49:56Z
• Authors: Robert A. Skinner; Sandra G. Hickmon; Charles K. Lumpkin; James Aronson; Richard W. Nicholas
• Keywords: Bone decalcification

Producing quality slides of paraffin embedded, decalcified bone depends on the interaction several independent mechanisms that all must work in concert. Grossing, fixation, decalcification, processing, embedding, sectioning, and staining each comprise a unique procedure when examining slide. A system integrates these concepts proven workable formula is essential for specimen management necessary greater reproducibility. thorough understanding component to tailor variety hard tissue types specimens. (The J Histotechnol 20:267, 1997)