Pan-genomic analysis of bovine monocyte-derived macrophage gene expression in response to in vitro infection with Mycobacterium avium subspecies paratuberculosis

• DOI: 10.1186/1297-9716-43-25
• Publication Date: 2012-03-28T12:31:35Z
• Authors: David E MacHugh; Maria Taraktsoglou; Kate E Killick; Nicolas C Nalpas; John A Browne; Stephen DE Park; Karsten Hokamp; Eamonn Gormley; David A Magee
• Keywords: Time Factors; Veterinary medicine; Research; Gene Expression Profiling; Macrophages; 04 agricultural and veterinary sciences; veterinary(all); Polymerase Chain Reaction; 3. Good health; Mycobacterium avium subsp. paratuberculosis; 0403 veterinary science; Gene Expression Regulation; SF600-1100; Host-Pathogen Interactions; Paratuberculosis; Animals; Cattle; Female; Oligonucleotide Array Sequence Analysis

Mycobacterium avium subspecies paratuberculosis is the causative agent of Johne's disease, an intestinal disease ruminants with major economic consequences. Infectious bacilli are phagocytosed by host macrophages upon exposure where they persist, resulting in lengthy subclinical phases infection that can lead to immunopathology and dissemination. Consequently, analysis macrophage transcriptome response M. subsp. provide valuable insights into molecular mechanisms underlie disease. Here, we investigate pan-genomic gene expression bovine monocyte-derived (MDM) purified from seven age-matched females, vitro (multiplicity 2:1) at intervals 2 hours, 6 hours 24 post-infection (hpi). Differentially expressed genes were identified comparing transcriptomes infected MDM non-infected control each time point (adjusted P-value threshold ≤ 0.10). 1050 differentially unique hpi, 974 78 detected respectively. Furthermore, number upregulated exceeded downregulated point, fold-change for markedly higher than genes. Inspection systems biology revealed enrichment involved inflammatory response, cell signalling pathways apoptosis. The transcriptional changes associated cellular may reflect different immuno-modulatory host-pathogen interactions during infection.