PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori

• DOI: 10.1186/1471-2180-11-101
• Publication Date: 2011-05-14T18:16:23Z
• Authors: Laura Cerqueira; Ricardo M Fernandes; Rui M Ferreira; Fátima Carneiro; Mário Dinis-Ribeiro; Céu Figueiredo; Charles W Keevil; Nuno F Azevedo; Maria J Vieira
• Keywords: Peptide nucleic acid; Gold standard (test); Helicobacter Infections

Abstract Background Triple therapy is the gold standard treatment for Helicobacter pylori eradication from human stomach, but increased resistance to clarithromycin became main factor of failure. Until now, fastidious culturing methods are generally method choice assess status. In this study, a new genotypic detect in clinical samples, based on fluorescent situ hybridization (FISH) using set peptide nucleic acid probes (PNA), proposed. Results The targeting point mutations responsible was applied H. suspensions and showed 100% sensitivity specificity (95% CI, 79.9-100 95% 71.6-100 respectively). This can also be amenable application gastric biopsy as detected when histological slides were tested. Conclusions optimized PNA-FISH diagnostic shown very sensitive specific detection smears proved reliable diagnosis pathogen samples an alternative existing plating methods.