Intragenomic diversity of Rhizobium leguminosarum bv. trifolii clover nodule isolates

• DOI: 10.1186/1471-2180-11-123
• Publication Date: 2011-05-31T02:18:46Z
• Authors: Andrzej Mazur; Grażyna Stasiak; Jerzy Wielbo; Agnieszka Kubik-K...; Monika Marek-Koza...; Anna Skorupska
• Keywords: Rhizobium leguminosarum; Parasitology

Soil bacteria from the genus Rhizobium are characterized by a complex genomic architecture comprising chromosome and large plasmids. Genes responsible for symbiotic interactions with legumes usually located on one of plasmids, named plasmid (pSym). The plasmids have great impact not only metabolic potential rhizobia but also underlie genome rearrangements plasticity.Here, we analyzed distribution sequence variability markers chromosomes extrachromosomal replicons leguminosarum bv. trifolii strains originating nodules clover grown in same site cultivated soil. First, basis similarity repA repC replication genes to respective counterparts chromids reported R. viciae 3841 etli CFN42, chromid-like were distinguished pool nodule isolates studied. Next, gene content was different compartments, i.e., chromosome, 'other plasmids'. stable unstable chromosomal detected hybridization data. Displacement few between plasmids', as well loss some observed sampled strains. Analyses chosen sequences allowed estimation degree their adaptation three compartments host.Our results showed that differences divergence can be even within small group recovered clovers at site. Substantial organization could especially taking into account DNA. Despite high concerning number size among studied strains, conservation location dynamic individual (especially genes) particular compartment demonstrated. may affected given compartment. plasmid' less adapted host than genes.