Erythropoietin suppresses the activation of pro-apoptotic genes in head and neck squamous cell carcinoma xenografts exposed to surgical trauma

• DOI: 10.1186/1471-2407-14-648
• Publication Date: 2014-09-02T13:01:03Z
• Authors: Gustaf Lindgren; Lars Ekblad; Johan Vallon-Chri...; Elisabeth Kjellén; Maria Gebre-Medhin; Johan Wennerberg
• Keywords: Surgical oncology

Several studies on the use of erythropoietin (Epo) to treat anaemia in patients undergoing cancer treatment have shown adverse effects tumour control and survival. Experimental indicate that this could be linked an interaction with wound healing processes not effect cells per se. We previously combination surgical trauma stimulates growth. In present study, we investigated surgery Epo gene expression. Human tumours from oral squamous cell were xenotransplanted nude mice treated Epo. The then transected a standardised procedure mimic change expression was by microarray analysis. qRT-PCR used measure levels mRNAs pro-apoptotic genes. frequency apoptosis assessed using immunohistochemistry for caspase-3. There little genes involved growth angiogenesis but significant down-regulation apoptosis. This confirmed general decrease mRNA selected Epo-treated had significantly lower as measured caspase 3. Our results suggest increased during might due inhibition apoptosis, becomes tissue damage such surgery. further suggests decreased survival