Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production

• DOI: 10.1186/1475-2859-4-2
• Publication Date: 2005-01-12T20:28:42Z
• Authors: Yves Le Loir; Vasco Azevedo; Sergio C Oliveira; Daniela A Freitas; Anderson Miyoshi; Luis G Bermúdez-H...; Sébastien Nouaille; Luciana A Ribeiro; Sophie Leclercq; Jane E Gabriel; Valeria D Guimaraes; Maricê N Oliveira; Cathy Charlier; Michel Gautier; Philippe Langella
• Keywords: Heterologous; Proteolysis; Secretory protein

Lactococcus lactis, the model lactic acid bacterium (LAB), is a food grade and well-characterized Gram positive bacterium. It good candidate for heterologous protein delivery in foodstuff or digestive tract. L. lactis can also be used as producer fermentor. Many proteins have already been produced but only few reports allow comparing production yields given either intracellularly secreted medium. Here, we review several works evaluating influence of localization on lactis. The questions size limits, conformation, proteolysis are addressed discussed with regard to yields. These data show that i) secretion preferable cytoplasmic production; ii) enhancement (by signal peptide propeptide optimization) results increased yield; iii) conformation rather than impair thus alter yields; iv) fusion stable stabilize labile proteins. role intracellular precursors discussed. new challenges now development systems identification optimization host factors affecting not other LAB species.