Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004

• DOI: 10.1186/1751-0147-53-38
• Publication Date: 2011-06-15T07:09:11Z
• Authors: Tamie Noda; Koichi Murakami; Tetsuo Asai; Yoshiki Etoh; Tomoe Ishihara; Toshiro Kuroki; Kazumi Horikawa; Shuji Fujimoto
• Keywords: Multilocus sequence typing; Housekeeping gene; Salmonella enteritidis; Salmonella enterica; Phage typing; Lineage (genetic)

Abstract Salmonella enterica subsp. serovar Enteritidis ( S . Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in dominant lineage before after this event remain unknown. This study determined lineages Japan using multilocus sequence typing (MLST). Thirty strains were collected between 1973 2004, consisting of 27 human from individual episodes, bovine strain, liquid egg strain an eggshell strain. Strains showed nine phage types 17 pulsed-field profiles with gel electrophoresis. All had homologous type 11 sequences without any nucleotide differences seven housekeeping genes. These MLST results suggest that diversities revealed by profiling has highly clonal population. Although may exhibit both pleiotropic surface structure variation, it is likely to be stable derived ancestor and/or 1990s Japan.