Distinct synovial immunopathology in Behçet disease and psoriatic arthritis

• DOI: 10.1186/ar2608
• Publication Date: 2009-02-07T08:19:46Z
• Authors: Juan D Cañete; Raquel Celis; Troy Noordenbos; Conchita Moll; Jose A Gómez-Puerta; Pilar Pizcueta; Antonio Palacin; Paul P Tak; Raimon Sanmartí; Dominique Baeten
• Keywords: Inflammation; Knee Joint; Neutrophils; Behcet Syndrome; T-Lymphocytes; Immunology; Arthritis, Psoriatic; Synovial Membrane; 610; Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; 3. Good health; Arthroscopy; 03 medical and health sciences; 0302 clinical medicine; Rheumatology; Synovectomy; 616; Synovial Fluid; Image Processing, Computer-Assisted; Immunology and Allergy; Cytokines; Humans; Research Article

Abstract Introduction The aim of the study was to investigate synovial immunopathology differences between early Behçet disease (BD) and psoriatic arthritis (PsA). Methods Needle arthroscopy an inflamed knee joint performed in patients with untreated BD (n = 8) PsA 9). Synovial fluid (SF) collected for cytokines, perforin, granzyme analysis. Eight biopsies per patient were obtained immunohistochemical analysis cellular infiltrate (T cells, natural killer macrophages, B plasma mast neutrophils), blood vessels as well expression perforin granzyme. stained slides evaluated by digital image Results global degree inflammation similar two types arthritis. In innate immune cell infiltration, there a striking neutrophilic synovitis whereas displayed significantly higher numbers cells positive c-kit, marker cells. As lymphocytes, CD3 + T but neither CD20 nor CD138 increased versus PsA. Further T-lymphocyte population showed no clear shift CD4/CD8 ratio or Th1/Th2/Th17 profile. SF levels effector molecule cytotoxic significant four- fivefold increase BD. Conclusions This systematic comparative identifies neutrophils lymphocytes important infiltrating populations Increased suggest relevance cytotoxicity this disease.