Analysis of IgG4 class switch-related molecules in IgG4-related disease

• DOI: 10.1186/ar3924
• Publication Date: 2012-07-23T12:13:49Z
• Authors: Hiroto Tsuboi; Naomi Matsuo; Mana Iizuka; Sayaka Tsuzuki; Yuya Kondo; Akihiko Tanaka; Masafumi Moriyama; Isao Matsumoto; Seiji Nakamura; Takayuki Sumida
• Keywords: IgG4-Related Disease

Abstract Introduction Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a new entity characterized by high serum IgG4 levels, IgG4-positive plasmacytic infiltration, and fibrosis in various organs. The purpose of this study was to determine the mechanism upregulation class switch recombination IgG4-RD. Methods We extracted RNA from peripheral blood mononuclear cells (PBMCs) patients with IgG4-RD ( n = 6), Sjögren syndrome (SS) healthy controls 8), CD3-positive T CD20-positive B sorted PBMCs 3), SS 4), as well labial salivary glands (LSGs) 11), 13), 3). mRNA expression levels IgG4-specific switch-related molecules, such Th2 cytokines (IL-4 IL-13), Treg (IL-10 TGF-β), transcriptional factors (GATA3 Foxp3) were examined quantitative polymerase chain reaction (PCR). IgG4-nonspecific CD40, CD154, BAFF, APRIL, IRF4, AID, also examined. Results TGF-β) AID significantly higher LSGs than P < 0.05, each). In contrast, those CD40 CD154 lower each), whereas comparable three groups. Conclusion Overexpression IL-10, TGF-β, might play important roles pathogenesis IgG4-RD, class-switch fibrosis. seems be mainly upregulated affected