Comparison of commercial DNA extraction kits for the detection of bacterial genomic DNA from whole-blood samples using a broad-range PCR

• DOI: 10.1186/cc8069
• Publication Date: 2009-11-11T14:15:00Z
• Authors: B Krulova; E Nemcova; B Zaloudikova; P Nemec; T Freiberger
• Keywords: genomic DNA; Gold standard (test)

Introduction Urokinase receptor (uPAR, CD87), a glycosylphosphatidylinositol-anchored protein, is considered to play an important role in inflammation and fibrinolysis.The Gram-negative bacterium Burkholderia pseudomallei able survive replicate within leukocytes causes melioidosis, cause of pneumonia-derived community-acquired sepsis Southeast Asia.We here investigated the expression function uPAR both patients with septic melioidosis murine model experimental melioidosis.Methods Using translational approach we conducted patient study culture-confirmed caused by B. pseudomallei, vitro experiments using wild-type (WT) knockout (KO) cells, mouse studies WT KO mice inoculated pseudomallei.Results mRNA surface was increased in/on peripheral blood monocytes granulocytes as well pulmonary compartment during mice.uPAR-deficient intranasally infected showed enhanced growth dissemination when compared mice, corresponding hepatic inflammation.uPAR demonstrated significantly reduced neutrophil migration towards after inoculation pseudomallei.Further that uPARdeficient macrophages display markedly impaired phagocytosis pseudomallei.Additional deficiency did not influence hemostatic fibrinolytic responses severe melioidosis.Conclusions These data suggest crucially involved host defense against facilitating neutrophils primary site infection subsequently pseudomallei.P2 A comparison acute lung Klebsiella pneumoniae B5055-induced pneumonia BALB/c