Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma

• DOI: 10.1186/s11658-018-0070-8
• Publication Date: 2018-04-27T11:23:28Z
• Authors: Jing Liu; Chunxia Yang; Yufang Gu; Chong Li; Huamei Zhang; Wenfang Zhang; Xueqing Wang; Nan Wu; Chunyan Zheng
• Keywords: Male; Epstein-Barr Virus Infections; Cell Survival; Mice, Nude; Apoptosis; SNHG8; Cell growth; Mice; 03 medical and health sciences; shRNA; Stomach Neoplasms; Cell Line, Tumor; Animals; Humans; RNA, Small Interfering; Cell Proliferation; Mice, Inbred BALB C; 0303 health sciences; QH573-671; Research; Cell Cycle; Xenograft Model Antitumor Assays; 3. Good health; Epstein-Barr virus-associated gastric carcinoma; RNA, Long Noncoding; Cytology

Epstein-Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one the highest mortality rates all cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in GC. SNHG8 recently identified lncRNA that was reported to abnormal expression pattern However, little known its biological function EBV-associated GC.We used cell viability, colony formation and cycle assays investigate growth GC.The transcript levels cultured GC cells were significantly higher compared normal mucosal EBV-negative cells. Knockdown specific shRNAs inhibited proliferation arrested G0/G1 phase vitro. We also found knockdown suppressed tumor vivo.These data indicate pro-oncogenic potential GC, meaning it latent therapeutic target for treatment this type cancer.