Salivary Electrochemical Cortisol Biosensor Based on Tin Disulfide Nanoflakes

• DOI: 10.1186/s11671-019-3012-0
• Publication Date: 2019-06-04T13:02:59Z
• Authors: Xinke Liu; Sanford P. C. Hsu; Wai-Ching Liu; Yi-Min Wang; Xinrui Liu; Ching-Shu Lo; Yu-Chien Lin; Sasza Chyntara Na...; Zhiwen Li; Yuehua Hong; Chingpo Lin; Yunqian Li; Gang Zhao; Ren-Jei Chung
• Keywords: Differential pulse voltammetry; Bovine serum albumin; Glassy carbon

Cortisol, a steroid hormone, is secreted by the hypothalamic-pituitary-adrenal system. It well-known biomarker of psychological stress and hence known as "stress hormone." If cortisol overexpression prolonged repeated, dysfunction in regulation eventually occurs. Therefore, rapid point-of-care assay to detect needed. Salivary electrochemical analysis non-invasive method that potentially useful enabling measurement levels. In this study, multilayer films containing two-dimensional tin disulfide nanoflakes, antibody (C-Mab), bovine serum albumin (BSA) were prepared on glassy carbon electrodes (GCE) BSA/C-Mab/SnS2/GCE, characterized using impedance spectroscopy cyclic voltammetry. Electrochemical responses biosensor function concentrations determined voltammetry differential pulse This exhibited detection range from 100 pM μM, limit pM, sensitivity 0.0103 mA/Mcm2 (R2 = 0.9979). Finally, authentic saliva samples obtained developed system correlated well with results enzyme-linked immunosorbent assays. was successfully used for salivary over physiological ranges, based specificity antibody-antigen interactions.