Expansion of Single Cell Transcriptomics Data of SARS-CoV Infection in Human Bronchial Epithelial Cells to COVID-19

• DOI: 10.1186/s12575-020-00127-3
• Publication Date: 2020-07-23T15:04:04Z
• Authors: Reza Zolfaghari E...; Hassan Nosrati; Mahyar Eftekhari; Reza Falak; Majid Khoshmirsafa
• Keywords: 2019-20 coronavirus outbreak

Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of disease 19 (COVID-19) that was emerged as a new member coronaviruses since December 2019 in Wuhan, China and then after spread all continentals. Since SARS-CoV-2 has shown about 77.5% similarity to SARS-CoV, transcriptome immunological regulations expected have high percentage overlap with SARS-CoV. Results In this study, we applied single cell transcriptomics data human bronchial epithelial cells (2B4 line) infected which annotated Expression Atlas database expand COVID-19. addition, employed system biology methods including gene ontology (GO) Reactome pathway analyses define functional genes pathways The analysis on revealed most from 2B4 line SARS-CoV were downregulated leading immune hyperactivation, induction signaling pathways, consequently cytokine storm. GO:0016192 (vesicle-mediated transport), GO:0006886 (intracellular protein GO:0006888 (ER Golgi vesicle-mediated transport) top three GOs network Meanwhile, R-HAS-6807070 (phosphatase tensin homolog or PTEN regulation) showed highest association other plays critical role activation dendritic cells, B- T-cells, secretion proinflammatory cytokines, cooperates promotion storm COVID-19 patients. Conclusions Based SARS-CoV-2, regulations, cytokines infection can be expanded valid platform for future pharmaceutical vaccine studies.